The facility has a range of light microscopes on the St James's University Hospital campus providing high resolution, fluorescence, high throughput-high content and live cell imaging monitoring capabilities. The facility currently houses 6 imaging systems and is constantly looking to resource new technologies that can help to improve our understanding of cell biology. All our equipment can be readily reserved thorough the QReserve online calendar system. Offline analysis of data can be carried out on any of our dedicated offline data analysis PC free of charge. All our microscopes are user-operated following specific training for the instrumentation from facility support staff. To organise training on a system please email: email@example.com.
Laser Scanning Confocal Microscopes (LSM)
A1R LSM (Nikon Instruments) – Room 8.04j WTBB
The A1R is our most capable system built on the Eclipse TiE Microscope (Nikon Instruments) providing stable time-lapse imaging with automatic focus correction system and a high speed motorized stage with a broad range of sample vessel options. The A1R LSM provides a hybrid scan head allowing for the collection of high-quality images through its unique pinhole and galvano scan head or high acquisition rates with 512 x 512-pixel definition at speeds of up to 30 frames per second. This system is fitted with its own incubation chamber allowing for live cell confocal assessments in gas and temperature controlled environment. The system has enhanced spectral imaging abilities and is able to perform fast acquisition of 32-channel spectral images and real-time spectral unmixing. Additional key features of this system include the incorporation of a perfect focus system and a fast piezoelectric focus drive. The microscope is equipped with 405nm, 457-514nm, 561nm and 642nm lasers. The system is controlled by the easy to use NIS-Elements C advanced software platform which enables complicated sequences of experiments to be performed such as photoactivation, image tiling (stitching of adjacent fields to give high resolution imaging of tissues) and multidimensional image acquisition (x, y, z, time, wavelength, and multipoint). For details click: https://sites.google.com/view/flowimaginguol/imaging/imaging-equipment/laser-scanning-confocal
C1 LSM (Nikon Instruments) Room 8.04j WTBB
The C1 is our workhorse confocal LSM based on the TE2000E inverted microscope (Nikon Instruments) providing stable time-lapse imaging with automatic focus correction system and an ORCA ER monochromatic camera for TIRF (total internal reflection fluorescence) microscopy. The Nikon C1 four laser scanning confocal with TIRF attachment, housed in a heated CO2 incubation chamber. This system supports multichannel fluorescence capture, time-lapse recording, photoactivation imaging and spectral unmixing (For details click: https://sites.google.com/view/flowimaginguol/imaging/imaging-equipment/laser-scanning-confocal).
High Content High Throughput Fluorescence Microscopy
Operetta CLS (Perkin Elmer) Room 8.04d WTBB
With a choice of objectives and up to 4 channels per run the Operetta offers a way to collect images and data from 96 wells plates automatically with the option of a robotic plate handler for automatic running of large numbers of plates. Harmony and Columbus software offer meta-analysis capabilities (For details click: https://sites.google.com/view/flowimaginguol/imaging/imaging-equipment/throughput-highcontent-imaging).
Widefield Fluorescence Microscopes
Eclipse TiE (Nikon instruments) Room 8.04f WTBB
The Eclipse TiE Microscope (Nikon Instruments) widefield fluorescence microscope is a workhorse within the facility allowing for high-quality phase, chromophore and fluorescence assessments of cell lines, and tissue in a wide variety of vessels. Often this is used to more cheaply optimise research approaches prior to moving these projects onto more expensive and complex technologies such as confocal microscopy (For details click: https://sites.google.com/view/flowimaginguol/imaging/imaging-equipment/widefield-fluorescence-micrscopes).
Live Cell Imaging Microscopes
BioStation IM (Nikon Instruments) Room 8.04 WTBB
The BioStation IM Microscope (Nikon Instruments) widefield fluorescence live cell imaging microscope is a workhorse within the facility allowing for high-quality phase, and fluorescence assessments of cell lines over long time courses requiring a controlled atmosphere and high optical magnifications. Often this is used to more cheaply optimise research approaches and experimental time frames prior to moving these projects onto more expensive and complex technologies such as confocal microscopy looking at a more focused time course (For details click: https://sites.google.com/view/flowimaginguol/imaging/imaging-equipment/live-cell-imaging-systems).
IncuCyte Zoom (Sartorius) Room 9.04c WTBB
The IncuCyte Zoom (Sartorius) widefield fluorescence live cell imaging microscope is a workhorse within the facility allowing for high-quality phase, and fluorescence assessments of cell lines over time courses under a controlled atmosphere. Often this is used to more cheaply optimise research approaches prior to moving these projects onto more expensive and complex technologies such as confocal microscopy (For details click: https://sites.google.com/view/flowimaginguol/imaging/imaging-equipment/live-cell-imaging-systems).
Fluorescence, Absorbance & Luminescence Imaging
Cytation 5 (Biotek) room 5.04b WTBB
The system offers multi-mode detection for slide and plate formats with drug/reagent injection capabilities for fluorescence, absorbance and luminescence. Upgradable to offer microscopy with multi-mode fluorescence and phase outputs in z with environmental control. Gen5 Microplate Reader and Imaging Software allows for easy to use standard curve outputs from plate reader applications. Software includes functionality for easy image capture and analysis, for both qualitative and quantitative data including imaging deconvolution options. Capable of:
- Qualitative and quantitative assays, endpoint, kinetic
- EC50 determinations
- Parallel line analysis
- Spectral analysis
- Micro-volume direct nucleic acid analysis
For details click:
We offer our researchers and our external collaborators a range of services and support to allow a streamlined workflow from assay design through to publication. The core services offered by the FoMH core Flow Cytometry & Imaging Facility are:
- Project design
- Grant Costings
- Experimental Planning & Panel Design
- Applications Workshops
- Equipment Training
- Teaching Applications
- Data Analysis Support
- Experimental Troubleshooting
- Outputs for Data Publication
Offering a bespoke service tailored to your specific needs from a single plate to a whole library, whether you are looking to carry out your own screen or make use of our staff to carry out the work for you we have the expertise, equipment and experience to guide you through the whole screening process.
Please contact us to discuss your specific requirements.
We have a range of instruments available to facilitate the accurate, reproducible plate preparation for High Content work.
- For plate set-up there is an automated cell counter the Countess and for plating out cells fluidX xrd380 dispensers
- For the treatment of 96 well plates - adding ‘libraries’, etc. there is our liquid handling platform - Agilent Bravo
- The ‘fluidX xrd380’ dispensers are also available to facilitate and speed up staining protocols
Required Equipment and Facility Charges
- Combined Facility Annual Registration and access charge £50/yr
- Nikon A1 Confocal Microscope (LSM) - £29.84/hr (£24.10/hr 4 hr Discount Block Rate)
- Nikon TiE Widefield Fluorescent Microscope - £15.25/hr
- Nikon BioStation Fluorescent Live Cell Microscope (Dishes) - £2.79 /hr
- Essen IncuCyte Live Cell Microscope (Plates) - £3.16/hr
- Perkin Elmer Operetta High Through High Content Plate Microscope - £30.75/hr
- BioTek Cytation 5 Multi-Mode Imaging Plate Reader - £2.83/run
- Additional Staff Support for data Analysis or Acquisition on top of training or setting of instrument settings charge at £30/hr
For general enquiries and training requests please email the facility email: firstname.lastname@example.org
Dr Adam Davison
Imaging Facility Manager
Tel: (0113) 343 3768
Mrs Julie Higgins
Bioscreening & Imaging Research Technician
Tel: (0113) 343 8445
Mrs Liz Straszynski
Imaging Research Technician
Tel: (0113) 343 3768
Dr Jacquie Bond
Facility Academic Lead
More information can be found at the following links:
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