Alice V.R. Lake
- Position: Research Assistant
- Areas of expertise: CRISPR/Cas9; Genome Editing; Cilia; Microscopy; Reverse Genetics; siRNA screening; Molecular Genetics
- Email: A.V.R.Lake@leeds.ac.uk
- Phone: +44(0)113 343 8444
- Location: 8.11 Wellcome Trust Brenner Building, St James's University Hospital
- Website: Ciliopathy Research Group | Twitter | LinkedIn | Researchgate | ORCID
I'm an experienced biomedical Research Assistant currently working towards a Ph.D. in Molecular Genetics at The University of Leeds, St James's Hospital Campus. My main goal is to become a Technical Specialist to advance and promote the use of new technology within molecular biology. By sharing my expertise in biotechnologies I would be able to advise and aid others in achieving their specific research goals. Throughout my role as a Research Assistant, I have gained expertise in CRISPR/Cas9 genome-editing technology, High-Content experimental work and imaging, siRNA screening and Confocal Microscopy. I regularly share my expertise and specialist knowledge by delivering training to my colleagues and by mentoring junior scientists.
Alongside my research, I am also working as an Early Careers Group Representative for my Leeds Institute of Medical Research. My main duties include: Organising monthly seminars, workshops and lectures; providing a resource of information and links on personal career development; arranging external speakers to give first hand accounts and advice on alternative career options; promote work-life balance and stress management; make new collaborations with external contacts in industry and career coaching to ensure a diverse portfolio of events and resources.
2010 - 2014: MBiolSci Medical Genetics, Department of Molecular Biology and Biotechnology, University of Sheffield
2014 - Present: Research Assistant, Ciliopathy Research Group, Leeds Institute of Medical Research at St. James, University of Leeds
2015 - Present: Ph.D. Medicine (Part-Time), Ciliopathy Research Group, Leeds Institute of Medical Research at St. James, University of Leeds
2016 - 2018: Post-graduate Research Student Representative, Faculty of Medicine and Health and Leeds University Union
Providing technical support and teaching in the laboratory.
Coaching and mentoring Undergraduate, Master and early Ph.D level students through their research projects.
Maintaining the laboratory and managing resources for my research group.
Generating primary data for grant proposals.
Preparing data for publication or presentations.
- Early Careers Group Representative
Identifying new genes control the organisation and production of cilia
Developing cellular models of disease using CRISPR/Cas9 genome editing techniques
My research focuses on finding new genes and genetic interactions that are involved in maintaining primary cilia. Primary cilia are a small hair-like structure that protrude from cells and are a central hub for signalling pathways. My research can help us understand the molecular controls that organise cilia and thus contributes to understanding the pathology of ciliopathies (genetic disorders caused by mutations in cilia genes).
Other Research Interests
Using molecular biology and reverse genetics techniques to better understand cilia (A small hair-like signalling organelle on the membrane of cells).
Looking at genetic interactions that underpin the molecular organisation of the transition zone (a small region at the base of cilia).
Understanding the role of actin organisation in ciliogenesis (the formation of a cilium).
Advances in microscopy techniques
CRISPR/Cas9 genome editing technology
- MBiolSci Medical Genetics, 1st Class (Hons), University of Sheffield
- British Society of Cell Biology
- Biochemical Society
Research groups and institutes
- Leeds Institute of Medical Research at St James's